Biophysically Sorted Osteoprogenitors From Culture Expanded Bone Marrow Derived Mesenchymal Stromal Cells (MSCs)

摘要

The invention provides, inter alia, populations of large mesenchymal stem cells (MSC)(as well as conditioned medium from these cells) with enhanced regenerative potential, as well as methods of culturing and using these populations, such as therapeutic methods of mediating tissue repair or enhancing homing and engraftment of hematopoietic stem cells. These large MSC populations can, in certain embodiments, be produced by biophysically sorting an MSC-containing population.

主权项

1. A method of producing an MSC population with enhanced regenerative potential, comprising:
a) culturing an MSC-containing population of cells in a culture medium to a confluence of 80% to 90% over each of at least four population doublings to provide a population enriched in large-MSC cells, wherein the four population doublings are measured from initial extraction of the MSC-containing population from a source; and b) biophysically sorting the MSC-containing population of cells produced in step (a) and collecting a large-MSC population of cells from the MSC-containing population, thereby producing an MSC population with enhanced regenerative potential.