METHODS AND REAGENTS THAT SPECIFICALLY DETECT, DISTINGUISH AND QUANTIFY IFN-LAMBDA2 mRNA FROM IFN-LAMBDA3 mRNA IN HUMANS

摘要

The present invention provides a method of specifically detecting IFN-λ2 mRNA or IFN-λ3 mRNA. There is provided a qRT-PCR method specifically detecting, discriminating and quantifying IFN-λ2 and IFN-λ3 mRNA in a biological sample obtained from a human. There is provided qRT-PCR methods and primers and probes that specifically detect IFN-λ2 mRNA but not IFN-λ3 mRNA and vice versa in humans in order to detect, quantify and discriminate IFN-λ2 mRNA and IFN-λ3 mRNA.

主权项

1. A kit suitable for quantifying IFN-λ2 mRNA, comprising:
a. a IFN-λ2-specific forward primer, said forward primer consisting of SEQ ID NO: 3; b. a IFN-λ2-specific reverse primer, said reverse primer consisting of SEQ ID NO: 4; c. a IFN-λ2-specific positive control, said positive control consisting of SEQ ID NO: 1; and d. a IFN-λ2-specific negative control, said negative control consisting of SEQ ID NO: 2; and e. an instruction for performing a qRT-PCR to specifically quantify IFN-λ2 in a biological sample.