发明名称 |
INTEGRATED PHOSPHOR NANOPARTICLE MARKING AGENT, AND FLUORESCENT IMMUNOSTAINING METHOD EMPLOYING SAME |
摘要 |
Provided are: a phosphor-integrated nanoparticle labeling agent which is capable of yielding a sufficient signal intensity even when its final concentration in an immunofluorescent staining reaction system is low (e.g., 0.02 nM) and enables to obtain an immunofluorescently stained image with reduced noise by inhibiting non-specific adsorption of a probe biological substance and a label to substances other than a detection subject; and an immunostaining method using the same. The phosphor-integrated nanoparticle labeling agent is a set which includes: a probe biological substance 3, which is linked to a first binding group substance A via a first hydrophilic polymer-derived spacer 1 having a length of 30 Å to 1,000 Å and specifically binds to a biomolecule 2; and a phosphor-integrated nanoparticle 5, which has a second binding group substance B capable of specifically binding to the first binding group substance A. |
申请公布号 |
EP3115783(A1) |
申请公布日期 |
2017.01.11 |
申请号 |
EP20150758624 |
申请日期 |
2015.03.04 |
申请人 |
Konica Minolta, Inc. |
发明人 |
GOUDA, Hideki;TAKAHASHI, Masaru |
分类号 |
G01N33/533;B82Y5/00;B82Y20/00;B82Y40/00;G01N33/53;G01N33/547 |
主分类号 |
G01N33/533 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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