Method for immunologically measuring soluble LR11

摘要

To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample).;The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.

主权项

1. A method for immunologically assaying soluble LR11 present in a biological sample selected from the group consisting of plasma, serum, cerebrospinal fluid and urine, the method comprising:
treating the sample with at least one surfactant selected from the group consisting of N-decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (SB10), N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (SB12), N-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (SB14), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO); reacting the treated sample with an anti-soluble LR11 antibody; and subjecting the reacted sample to an immunological assay.