Method to increase detection efficiency of real time PCR microarray by quartz material

摘要

A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

主权项

1. A reactor for the quantitative analysis of target nucleic acids, comprising:
a substrate having a first planar opposing surface and a second planar opposing surface, the first planar opposing surface of the substrate having a cavity; a buffer layer comprising a water-impermeable sealant arranged over the first planar surface of the substrate; a quartz cover plate modified with amino-silane and a functional group, wherein the cover plate is arranged over the buffer layer, the cover plate in combination with the cavity and buffer layer defining a reaction chamber; and nucleic acid probes tethered to the interior surface of the quartz cover plate in a known, two-dimensional pattern; wherein the quartz cover plate has less fluorescent background than optical glass k9 for quantitative analysis of target nucleic acids by an evanescent wave detection technique.