Use of antibodies for detection of IL-10

摘要

Provided is a humanized or chimeric antibody or fragment thereof capable of binding to interleukin-10 (IL-10), wherein said antibody or fragment thereof: (i) binds to the same region of IL-10 as the IL-10 receptor α (IL-I10Ra) and is not capable of binding IL-10 when the IL-10 is bound to the IL-10 receptor; and (ii) binds to IL-10 in homodimeric form by binding a discontinuous epitope comprising residues of both monomers. Further provided are related products and methods involving the use of the antibody or fragment thereof.

主权项

1. A method of detecting the level of soluble IL-10 homodimer in a sample, comprising: (a) contacting a sample with an antibody or fragment thereof; and (b) determining the amount of IL-10 present in the sample by determining the amount of antibody or fragment thereof bound to IL-10; wherein the antibody or fragment thereof is a humanized antibody or fragment thereof that binds to IL-10 in homodimeric form by binding a discontinuous epitope comprising residues of both monomers, and wherein said antibody or fragment thereof comprises a variable light chain comprising the amino acid sequences of CDR1, CDR2 and CDR3 of SEQ ID NO: 2, and a variable heavy chain comprising the amino acid sequences of CDR1, CDR2 and CDR3 of SEQ ID NO: 3, wherein the variable heavy chain further comprises the residues Asn73 and Ser74 of SEQ ID NO:70 which contact IL-10 when said antibody binds to IL-10.