Reagent cartridge and methods for detection of cells

摘要

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

主权项

1. An apparatus, comprising:
a housing configured to be removably coupled to a reaction chamber, the housing defining a reagent volume configured to receive a reagent container, the housing including a puncturer defining a transfer pathway in fluid communication with the reagent volume, the puncturer and the housing being monolithically constructed, the housing including a delivery portion defining a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber; and an actuator having a plunger portion disposed within the reagent volume, an engagement portion of the actuator configured to be manipulated to move the plunger portion within the reagent volume to deform the reagent container, the puncturer configured to pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and the delivery pathway.