| 主权项 |
1. A method for stabilizing an enzyme, which comprises:
storing the enzyme in a presence of a stabilized coenzyme, or a salt form thereof, or a reduced form thereof for a period of storage of at least 2 weeks at a temperature of at least 20° C., wherein the enzyme is selected from the group consisting of an alcohol dehydrogenase (EC 1.1.1.2), a glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and a diaphorase (EC 1.6.99.2), wherein the stabilized coenzyme is a compound of general formula (II):in whichA=adenine or an analogue thereof;T=in each case independently denotes O or S;U=in each case independently denotes OH, SH, BH3−, or BCNH2−;V=in each case independently denotes OH or a phosphate group, or two groups which form a cyclic phosphate group;W=COOR, CON(R)2, COR, or CSN(R)2 in which R in each case independently denotes H or a C1-C2 alkyl;X1, X2=in each case independently denote O, CH2, CHCH3, C(CH3)2, NH, or NCH3;Y=NH, S, O, or CH2;Z=a linear residue with 4-6 C atoms or a saturated or an unsaturated carbocyclic or heterocyclic 5-membered ring of general formula (III):in which a single or double bond may be present between R5′ and R5″, whereR4=in each case independently denotes H, F, Cl, or CH3;R5=CR42;R5′=O, S, NH, NC1-C2-alkyl, CR4, CHOH, or CHOCH3, and R5″=CR42, CHOH, or CHOCH3 if there is a single bond between R5′ and R5″;R5′=R5″=CR4 if there is a double bond between R5′ and R5″; andR6, R6′=in each case independently denote CH or CCH3;
wherein the Z and the pyridine residue are not linked by a glycosidic bond, and wherein enzyme activity decreases by less than 50% after the period of storage of at least 2 weeks at a temperature of at least 20° C. when compared to an initial value of enzyme activity. |
