Desorption electrospray ionization sampling without damaging an in vivo tissue sample

摘要

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

主权项

1. A method for analyzing in vivo tissue, the method comprising:
generating from a sampling probe, a liquid droplet spray discharge that is non-toxic to in vivo tissue; directing the liquid droplet spray discharge onto the in vivo tissue, wherein the liquid droplet spray discharge from the sampling probe desorbs and ionizes an analyte from the in vivo tissue in a manner that does not damage the in vivo tissue; transferring the ions of the analyte to a mass spectrometer; and analyzing the ions of the analyte in the mass spectrometer, thereby analyzing the in vivo tissue.