Method for increasing the efficiency of double-strand-break induced mutagenesis

摘要

The present invention relates to a method for increasing double-strand-break induced mutagenesis at a genomic locus of interest in a cell, thereby giving new tools for genome engineering, including therapeutic applications and cell line engineering. More specifically, the present invention concerns the combined use of TALEN or meganucleases with TREX2, especially under the form of single-chain proteins.

主权项

1. A method for increasing double-strand-break induced mutagenesis at a genomic locus of interest in a cell comprising the steps of:
(i) identifying at said genomic locus of interest at least one DNA target sequence cleavable by one natural or engineered rare-cutting endonuclease; (ii) expressing said rare-cutting endonuclease in the cell together with a polynucleotide encoding a fusion protein that comprises: a first polypeptide that is a human TREX2 exonuclease protomer of SEQ ID NO: 26 or functional mutant thereof that shares at least 80% identity with the human TREX2 exonuclease protomer; a second polypeptide that is a human TREX2 exonuclease protomer of SEQ ID NO: 26 or functional mutant thereof that shares at least 80% identity with the human TREX2 exonuclease protomer; and a peptidic linker connecting said first and second polypeptides; (iii) thereby obtaining, by expression of said fusion protein in said cell, increased double strand-break-induced mutagenesis at said genomic locus of interest.