| 摘要 |
A primer pair for detecting an idiopathic azoospermia-related genetic marker, the genetic marker being located in the coding region of the AR gene, and the nucleotide sequence thereof being represented by SEQ ID No.1, the mutation sites of the sequence being one or more of c. 868 T>C, c. 1484 G>A, c. 1888 C>T, c.569 C>T, c. 616 A>G, and c. 1149 C>T; the upstream primer and downstream primer of the primer pair are respectively located on the upstream and downstream of the mutation sites. By means of large-scale sequencing, 6 new mutation sites of the AR gene have been selected from idiopathic azoospermia patients; research demonstrates that said mutations of the AR gene may cause idiopathic azoospermia, and thus, by means of using the present primer pair to detect a mutation, diagnosis and detection of idiopathic azoospermia can be implemented. |
