| 摘要 |
In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described. |
