| 发明名称 |
DIRECT EVOLUTION OF A REGIOSELECTIVE HALOGENASE FOR INCREASED THERMOSTABILITY |
| 摘要 |
Compounds and methods are providing involving RebH variants with improved properties. directed evolution based on random mutagenesis was employed to generate a series of RebH variants. RebH variants with improved thermostability and increased activity at elevated temperatures were generated. |
| 申请公布号 |
US2017002334(A1) |
申请公布日期 |
2017.01.05 |
| 申请号 |
US201415100065 |
申请日期 |
2014.11.26 |
| 申请人 |
The University of Chicago |
发明人 |
Lewis Jared C.;Poor Catherine;Andorfer Mary;Payne James |
| 分类号 |
C12N9/02;C12P5/00 |
主分类号 |
C12N9/02 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. An isolated RebH variant polypeptide of SEQ ID NO: 1 comprising at least one amino acid substitution, wherein the at least one amino acid substitution results in improved halogenating activity; and wherein the at least one amino acid substitution is selected from the group consisting of S2P; I52T; A58V; M71V, M71T, M71A, or M71C; N75K; E96V; D101G; S110P or S110L; F111S; G112S or G112D; L113D or L113N; L114P; S130L; K145M or K145R; N166S; F171I, K187R; D203A or D203G; T213A; V225I; K237E, V256I; T258A; D264G; T283A; L289P; F312L; T322I; T348A; L380F; T394M; F396Y or F396L; R400C; T413A; E423D; A442V; S448P; L453P; F458S or F458L; E461G; F465C or F465L; N467T; N470S; A476T or A476V; V481A; Q494R; T496R or T496A; G504S; and R509Q. |
| 地址 |
Chicago IL US |
