METHOD FOR PREPARING TISSUE, PARTICULARLY ADIPOSE TISSUE, FOR TRANSPLANTATION FROM LOBULAR FAT EXTRACTED BY LIPOSUCTION

摘要

A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and of a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of washing the cell aggregates carried out at the same time as a step of separating the fluid component, in emulsion form, from the solid component.

主权项

1. A method of preparing adipose tissue for transplantation from lobular fat material extracted from a body by liposuction, said fat material comprising (a) a fluid component comprising one or more of an oily component, a blood component and sterile solutions, and (b) a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, said method comprising
washing the solid component at least once, and separating the fluid component, in emulsion form, from the solid component, wherein said washing and separating steps are carried out at the same time, and wherein said washing and separating steps include: injecting at least one sterile washing solution into a washing chamber containing the fat material, of a washing and separating container, wherein the washing chamber contains an outlet and at least one stirring element; manually or mechanically stirring said washing and separating container to facilitate the emulsion of the fluid components; arranging the washing and separating container in a vertical position relative to the ground, with the outlet facing downwards, to obtain a stratification of the solid components on the liquid emulsion, which constitutes the fat material contained in the washing chamber, which results in a solid component, composed of cell fragments, cells and one or more cell agglomerates, floating on an emulsion of the fluid components that are present in the lower portion of the washing chamber and in contact with said outlet of the washing and separating container; and discharging of the emulsion of fluid components from the washing chamber through the outlet of the washing and separating container.