发明名称 |
METHOD FOR HIGH SENSITIVITY DETECTION OF MYD88 MUTATIONS |
摘要 |
A method for increasing sensitivity for detecting minority mutations in MYD88 uses a locked nucleic acid oligo to block amplification of wild-type DNA in DNA isolated from patient FFPE tissue, bone marrow aspirate or peripheral blood samples during PCR while still allowing sequencing and visualization of the PCR product. Further improvement to the sensitivity may be achieved by using a uracil DNA-glycosylase treatment to remove sequence artifacts commonly found in formalin-fixed, paraffin-embedded tissue. |
申请公布号 |
WO2017004599(A1) |
申请公布日期 |
2017.01.05 |
申请号 |
WO2016US40869 |
申请日期 |
2016.07.01 |
申请人 |
NEOGENOMICS LABORATORIES, INC. |
发明人 |
ALBITAR, Maher |
分类号 |
C12P19/34;C12Q1/68;G01N33/48 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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