APPARATUS AND METHOD FOR PERFORMING NUCLEIC ACID ANALYSIS

摘要

The present invention relates to optical confinements, methods of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.

主权项

1. A method of sequencing a nucleic acid segment, comprising:
a) performing a single molecule nucleic acid sequencing reaction on a template nucleic acid molecule comprising a nucleic acid segment of interest, wherein the template nucleic acid molecule provides for iterative sequencing of the nucleic acid segment of interest, thereby generating multiple sequence reads of the nucleic acid segment of interest; and b) analyzing the multiple sequence reads to determine a nucleic acid sequence for the nucleic acid segment of interest.