摘要 |
Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. Other aspects of the methods use a phage conjugate as the detection moiety. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. |
申请人 |
ABBOTT LABORATORIES |
发明人 |
HUFF, Jeffrey B.;HAYDEN, Mark A.;KARABATSOS, Peter J.;SCHAPALS, Andrew S.;MUERHOFF, Anthony S.;BOGDAN, Maria Felicia;LEARY, Thomas;HOLETS-MCCORMACK, Shelley R.;LAURENSON, Sophie;FISCHER, Andrew T.;HAACK, Richard;HERSHBERGER, Stefan;HOUSE, Dustin;QIAO, Lei;MURPHY, M. Shawn;POPE, Mark R.;PRIETO-BALLENGEE, Edna M.;RUAN, QiaoQiao;SONI, Pathik;TETIN, Sergey;YARNELL, Lyle |