Synthetic Promoters

摘要

CHO cell-specific synthetic promoter constructs for expressing recombinant proteins, a library of promoter constructs thereof, and a method for producing the promoter constructs. The promoter constructs enable precise control of recombinant gene transcription over three orders of magnitude, with the top expressing promoters capable of double the transcriptional activity of the CMV promoter.

主权项

1. A CHO cell, comprising a synthetic promoter suitable for eliciting recombinant protein expression therein, said synthetic promoter comprising a promoter core and upstream thereof two or more transcription factor regulatory elements independently selected from the group consisting of NFκB-RE, E-box, AP1, CRE, GC-Box, E41F, C/EBPα-RE, OCT and RARE.