发明名称 |
COMPOSITIONS AND METHODS FOR SITE DIRECTED GENOMIC MODIFICATION |
摘要 |
The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage. |
申请公布号 |
EP3110945(A1) |
申请公布日期 |
2017.01.04 |
申请号 |
EP20150755923 |
申请日期 |
2015.02.27 |
申请人 |
Monsanto Technology LLC |
发明人 |
BROWER-TOLAND, Brent;KOURANOV, Andrei, Y.;KUEHN, Rosemarie;LAWRENCE, Richard, J.;NAGY, Ervin, D.;RYMARQUIS, Linda;VEENA, Veena |
分类号 |
C12N5/00;C07H21/02;C12N5/02;C12N15/00;C12N15/82;C12P19/34 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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