发明名称 |
ENRICHMENT OF DNA SEQUENCING LIBRARIES FROM SAMPLES CONTAINING SMALL AMOUNTS OF TARGET DNA |
摘要 |
Provided herein is a method for capturing DNA molecules in solution. The method may comprise: extracting DNA from a sample that comprises endogenous DNA and environmental DNA to produce extracted DNA; ligating universal adaptors to the extracted DNA; hybridizing the extracted DNA, in solution, with affinity-tagged RNA probes generated by: in vitro transcribing a library of fragmented reference genomic DNA that has been ligated to an RNA promoter adaptor, in the presence of an affinity-tagged ribonucleotide; binding the product with a capture agent that is tethered to a substrate in the presence of RNA oligonucleotides that are complementary to the adaptors, thereby capturing the hybridized DNA molecules on the substrate; washing the substrate to remove any unbound DNA molecules; and releasing the captured DNA molecules. A kit for performing the method is also provided. |
申请公布号 |
EP2992114(A4) |
申请公布日期 |
2017.01.04 |
申请号 |
EP20140794007 |
申请日期 |
2014.05.02 |
申请人 |
The Board of Trustees of the Leland Stanford Junior University |
发明人 |
BUSTAMANTE, Carlos D.;CARPENTER, Meredith L.;BUENROSTRO, Jason D.;GREENLEAF, William J. |
分类号 |
C12Q1/68;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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