Extra-capillary fluid cycling system and method for a cell culture device

摘要

An extra-capillary fluid cycling unit for maintaining and cycling fluid volumes in a cell culture chamber includes a housing and a first flexible reservoir extra-capillary fluid reservoir disposed in the housing. The extra-capillary fluid reservoir is in fluid communication with a cell culture chamber. A second flexible reservoir is also located in the housing, the second flexible reservoir being in fluid communication with a pressure source. A sensor plate is movably disposed in the housing between the extra-capillary reservoir and the second reservoir, wherein the second reservoir is pressurized to move the sensor plate in relation to the extra-capillary reservoir to cause fluid cycling and maintain fluid volumes in the cell growth chamber.

主权项

1. A fluid cycling unit for maintaining and cycling fluid volumes in a chamber comprising:
a housing; a first flexible fluid reservoir disposed in the housing, said first fluid reservoir being in fluid communication with a chamber; a second flexible reservoir located in said housing and adjacent said first reservoir, said second flexible reservoir being in fluid communication with a pressure source; a plate movably disposed in said housing between said first and second reservoirs, wherein said second reservoir is pressurized to move said plate in relation to said first reservoir, contacting said first and second reservoirs, to cause fluid cycling and maintain fluid volumes in said chamber; a sensor indicator connected to said plate, wherein physical expansion and contraction of said first reservoir moves the position of said sensor indicator within said housing; and an optical sensor in communication with said pressure source and said sensor indicator, wherein the position of said sensor indicator drives said optical sensor to expand or reduce the pressure within said second reservoir.