Systems and methods to increase protein yield from recombinant manufacturing processes

摘要

Embodiments disclosed herein provide systems and methods that increase protein yield from recombinant manufacturing processes. The systems and methods treat used depth filters with bound proteins of interest as a stationary phase exchange resin to recapture bound protein of interest from the depth filter.

主权项

1. A method of increasing the amount of a protein of interest available for recovery from a sample containing other components and following a first purification step of the sample comprising:
passing the sample through a depth filter; creating a chromatographic column wherein the depth filter is treated as a stationary phase; exposing the depth filter to an elution buffer wherein exposure to the elution buffer releases the protein of interest from the depth filter for subsequent recovery, wherein the protein of interest is Factor VIII, and wherein the elution buffer has a conductivity of at least 20 mS/cm.