发明名称 |
Purification systems and methods |
摘要 |
A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles. |
申请公布号 |
US9534092(B2) |
申请公布日期 |
2017.01.03 |
申请号 |
US201213371264 |
申请日期 |
2012.02.10 |
申请人 |
Life Technologies Corporation |
发明人 |
Hinz Wolfgang;Jones Ryan;Light David |
分类号 |
B07B1/00;C08J3/12;C12N15/10 |
主分类号 |
B07B1/00 |
代理机构 |
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代理人 |
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主权项 |
1. A method of preparing hydrogel polymeric particles, the method comprising:
filtering a plurality of hydrogel polymeric particles through a first filter having a pore size at a first permeate velocity to provide a first subset of hydrogel polymeric particles in the permeate and having a first particle size distribution; and filtering the first subset of hydrogel polymeric particles through a second filter having the pore size at a second permeate velocity to provide a second subset of hydrogel polymeric particles in the retentate derived from the first subset of hydrogel polymeric particles and having a second particle size distribution narrower than the first particle size distribution; wherein the average particle size of the second subset of hydrogel polymeric particles is in a range of 0.3 micrometers to 1.5 micrometers; wherein the first and second permeate velocities are different. |
地址 |
Carlsbad CA US |