发明名称 |
Measuring apparatus and fluorescence measuring method |
摘要 |
A low-noise fluorescence image and a low-noise luminescence image are acquired even if a luminescent substance having a high luminescence level is used. Provided is a measuring apparatus including an illumination optical system that radiates excitation light coming from an excitation light source onto a specimen A, an image acquisition portion that acquires an image by measuring light generated at the specimen, and an image processing portion that, based on a first image, which is acquired by the image acquisition portion without radiating the excitation light, and a second image, which is acquired by the image acquisition portion while radiating the excitation light, generates a fluorescence image by removing a luminescence component from the second image. |
申请公布号 |
US9535007(B2) |
申请公布日期 |
2017.01.03 |
申请号 |
US201514801012 |
申请日期 |
2015.07.16 |
申请人 |
OLYMPUS CORPORATION |
发明人 |
Hattori Toshiyuki |
分类号 |
G01N21/64;G01N21/27 |
主分类号 |
G01N21/64 |
代理机构 |
Holtz, Holtz & Volek PC |
代理人 |
Holtz, Holtz & Volek PC |
主权项 |
1. A measuring apparatus comprising:
an illumination optical system that radiates excitation light coming from an excitation light source onto a specimen; an image acquisition portion that acquires an image by measuring light generated at the specimen; and an image processing portion that, based on a first image, which is acquired by the image acquisition portion without radiating the excitation light, and a second image, which is acquired by the image acquisition portion while radiating the excitation light, generates a fluorescence image by removing a luminescence component from the second image, wherein the first image is a luminescence image including only the luminescence component from a luminescent substance included in the specimen, and wherein the second image is a luminescence and fluorescence image including both the luminescence component and a fluorescence component. |
地址 |
Tokyo JP |