Methods for detecting human metapneumovirus

摘要

The present invention discloses specific human metapneumovirus monoclonal antibodies. The antibody is at least two-fold less reactive with non-human metapneumoviruses including, but not limited to, respiratory viruses or avian metapneumoviruses. Further, the antibody is at least two-fold more reactive with a human metapneumovirus (i.e., for example, Type A or Type B) than with non-human metapneumoviruses including, but not limited to, respiratory viruses or avian metapneumoviruses. Consequently, these novel antibodies are useful as a clinical diagnostic agent, especially when using fresh nasopharengeal aspirates. The invention also contemplates numerous diagnostic platforms that together with the novel antibodies can support economical, fast, and highly selective detection and identification of clinical inoculum samples.

主权项

1. A method for detection of infection by a metapneumovirus, comprising:
contacting a biological sample taken from an individual suspected of a metapneumovirus infection with a composition comprising one or more human metapneumovirus (hMPV)-specific monoclonal antibodies selected from the group consisting of an hMPV monoclonal antibody produced by C2C10, an hMPV monoclonal antibody produced by F4A1, and an hMPV monoclonal antibody produced by CB7F3.