Flow chamber analyte detection method

摘要

A flow chamber and method for detecting the presence of one more cell produced analytes under flow conditions. The flow chamber includes two compartments separated by a permeable membrane on which a plurality of cells may be positioned. The permeable membrane shields one or more analyte sensors positioned one compartment from the convective transport forces of a fluid flow within the other compartment to allow reliable and accurate detection of cell-produced analytes and determination of the concentration of cell-produced analytes.

主权项

1. A method for detecting an analyte using a flow chamber comprising:
positioning a plurality of cells within the flow chamber, wherein said flow chamber comprises;
a first compartment comprising a fluid inlet and a fluid outlet for allowing fluid to flow through said first compartment;a second compartment;a first analyte sensor positioned within said second compartment for detecting an analyte;a permeable membrane, wherein said permeable membrane comprises:
a first surface for receiving the plurality of cells, wherein the first surface is exposed to fluid flow in said first compartment; anda second surface in said second compartment,wherein said permeable membrane separates said first compartment from said second compartment;cycling a flow rate of a flow of fluid flowing between the fluid inlet and the fluid outlet in a direction substantially parallel to the first surface of the permeable membrane between a control flow rate and a plurality of different flow rates that are each higher than the control flow rate, wherein each of the higher flow rates exerts a different shear stress on the surface of the permeable membrane in the first compartment; anddetecting one or more analytes produced by said plurality of cells.