摘要 |
Producing dihydrochalcone, preferably phloretin, using a transgenic microorganism comprises: (iv) providing a transgenic microorganism comprising a nucleic acid segment (a) comprising a bacterial chalcone isomerase encoding gene and/or nucleic acid segment (a1) comprising a plant chalcone isomerase encoding gene, and a nucleic acid segment (b) comprising a bacterial enoate reductase encoding gene and (v) adding one or more flavanones, preferably naringin, and/or their precursors or derivatives, to the transgenic microorganism and culturing the transgenic microorganism. Producing dihydrochalcone, preferably phloretin, using a transgenic microorganism comprises: (iv) providing a transgenic microorganism comprising a nucleic acid segment (a) comprising a bacterial chalcone isomerase encoding gene and/or nucleic acid segment (a1) comprising a plant chalcone isomerase encoding gene, and a nucleic acid segment (b) comprising a bacterial enoate reductase encoding gene, (v) adding one or more flavanones, preferably naringin, and/or their precursors or derivatives, to the transgenic microorganism and culturing the transgenic microorganism and (vi) optionally isolating and purifying the dihydrochalcone. Independent claims are included for: (1) the transgenic microorganism comprising (a), (a1) and (b); (2) vector, preferably plasmid, comprising the transgenic microorganism containing (a), (a1) and (B); and (3) a host cell comprising one or more identical or different vectors. |
申请人 |
Symrise AG |
发明人 |
Hilmer, Dr. Jens-Michael;Gross, Egon;Krammer, Dr. Gerhard;Ley, Dr. Jakob Peter;Gall, Mechthild;Bornscheuer, Prof. Dr. rer. nat. Uwe;Thomsen, Maren;Peters, Christin;Jonczyk, Patrick;Beutel, Dr. rer. nat. Sascha;Scheper, Prof. Dr. rer. nat. Tomas |