| 摘要 |
Embodiments of the invention relate to a mixed bacteria producing surfactant and its screening method. According to at least one embodiment, there is provided a mixed bacteria producing biosurfactant composed of three kinds of strains: Pseudomonas stutzeri with preservation No. CCTCC AB 205091, Nocardioides ginsengagri with preservation No. CCTCC S2013441, and Bacillus licheniformis with preservation No. CCTCC AB 205141. The mixed bacteria is obtained by choosing the bacterial strain for oil production awaiting screening, activating and culturing it, getting the fermentation liquid in primary screen with blood plate method, and re-screening fermentation liquid in primary screen with oil drain method. This mixed bacteria synthesizes the advantages of the three strain, thus producing a biosurfactant to enhance oil recovery in the oilfield. The screening method of this mixed bacteria has a broad scope of applications, which effectively reduce the screening cost with high accuracy, provides strong selectivity, is a convenient process and easy to operate, and has a shorter cycle compared with the current technology. |
