METHOD FOR PURIFICATION OF BIOMASS OF DERMATOPHAGOIDES FARINAE MITES FROM NUTRIENT MEDIUM

摘要

The invention relates to the field of medicine, namely to allergology and can be used for obtaining purified from nutrient medium biomass of microscopic Dermatophagoides farinae mites, applied for obtaining allergen. The method for purification consists in cultivation of Dermatophagoides farinae mites on powder of Daphnia. Growing was carried out in closed 500 ml beakers. Cultivation is carried out until complete exhaustion of nutrient medium with the following transfer of the entire mite mass into the next beaker without nutrient medium for a week. The procedure is repeated 4 times (4 weeks). The disclosed method was applied at the laboratory for production of allergen from Dermatophagoides farinae mites and can be used for purification of mite biomass in order to obtain initial biomaterial without admixtures of nutrient medium.