Kinase substrate sensor

摘要

The present invention relates to a cytoplasmic protein complex comprising (a) a first recombinant fusion protein comprising a kinase, fused to a first interaction polypeptide and (b) a second recombinant fusion protein comprising a domain comprising a reporter phosphorylation site, whereby said domain is fused to a second interaction polypeptide. The present invention relates further to a method to detect compound-compound-interaction using said cytoplasmic protein complex, and to cells comprising such cytoplasmic protein complex.