NANOMETER MIMIC ENZYME IMMUNOCHROMATOGRAPHY DETECTION METHOD

摘要

This invention provides a nanozyme immunochromatographic detection method for detecting a substance to be tested in a liquid sample, comprising, in this order, the steps of:1) providing a detection probe, which is prepared by coupling a magnetic nanoparticle to a first molecule capable of specifically binding to the substance to be tested;2) providing a capture probe, which is an immobilized second molecule capable of specifically binding to the substance to be tested;3) bringing the liquid sample into contact with the detection probe;4) bringing the liquid sample, which has been in contact with the detection probe, into contact with the capture probe; and 5) adding a hydrogen-donor substrate and a peroxide to the capture probe subject to the step 4) so as to perform color development reaction. This invention further provides a nanozyme immunochromatographic detection apparatus for detecting a substance to be tested in a liquid sample.