发明名称 |
STERILE CHROMATOGRAPHY RESIN AND USE THEREOF IN MANUFACTURING PROCESSES |
摘要 |
Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein. |
申请公布号 |
EP3094392(A1) |
申请公布日期 |
2016.11.23 |
申请号 |
EP20150702074 |
申请日期 |
2015.01.16 |
申请人 |
GENZYME CORPORATION |
发明人 |
GODAWAT, RAHUL;WARIKOO, VEENA;PATIL, ROHAN;KONSTANTINOV, KONSTANTIN;RYAKALA, VENKAT KISHORE |
分类号 |
B01D15/18;A61L2/08;B01D15/20 |
主分类号 |
B01D15/18 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|