| 摘要 |
A method for generating all coverage of valid primer pairs, which satisfy various filtering constraints provided by users with respect to a given sequence database, including genome level sequences, and has validated specificity to given sequences, is provided. The constraints can include length, melting temperature, GC content and self-complementarity, among others. All suitable primer pairs present on a given DNA sequence database are screened without omitting any one primer pair. All primers having a coverage of 1 or more as well as primers having a coverage of 1 can be screened. The primers are also scored and ranked, and may be used in experiments such as qPCR. |
