METHOD FOR THE INDUSTRIAL PURIFICATION OF BIOLOGICALLY ACTIVE PHYCOTOXINS

摘要

This invention is related to a method for the industrial purification of biologically active phycotoxins that comprises providing a suitable amount of a phycotoxin source; if the phycotoxin source is a pellet, said pellet is lysed using suitable methods; the material from the cyanobacterial lysis is subjected to a cold extraction; obtaining a concentrate of the aqueous phase obtained in the previous step or the culture medium of the first step; centrifugating the concentrate to obtain a supernatant; passing the supernatant through a diatomaceous earth column and obtaining a phycotoxin-containing eluate; passing the eluate obtained in the previous step through activated charcoal columns; the eluate from the previous step is passed again through a diatomaceous earth column; the eluate from the previous step is left in an aqueous phase; the partially purified extract from the previous step is subjected to a preparative HPLC in several steps to obtain the pure biologically active phycotoxin.