一种稳定、抗干扰能力强的血清腺苷脱氨酶检测试剂及检测方法

摘要

本发明涉及血清腺苷脱氨酶检测技术领域，特别涉及一种血清腺苷脱氨酶检测试剂，R1中含有缓冲液，4‑氨基氨替吡啉，PNP，XOD，POD，胆红素氧化酶，抗坏血酸氧化酶，丙三醇，聚乙二醇6000，乙二醇，甘露醇，海藻糖，BSA，烷基糖苷，防腐剂；R2中含有缓冲液，腺苷，3‑羟基‑2,4,6‑三碘苯甲酸，丙三醇，聚乙二醇6000，乙二醇，甘露醇，海藻糖，BSA，烷基糖苷，防腐剂；采用新的Trinder反应色原物质3‑羟基‑2,4,6‑三碘苯甲酸，添加多种稳定剂，显著改善了试剂稳定性；通过添加胆红素氧化酶、抗坏血酸氧化酶和新型非离子表面活性剂烷基糖苷（APG），有效避免了胆红素和抗坏血酸的干扰，防止反应体系浑浊，底物稳定性、试剂的抗干扰能力均增强。

主权项

一种血清腺苷脱氨酶检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：1）试剂R1的组分为：缓冲液_{·························}100mmol/L，4‑氨基氨替吡啉_{·················}5mmol/L，PNP（嘌呤核苷磷酸化酶）_{··········}1.5KU/L，XOD（黄嘌呤氧化酶）_{··············}3.1KU/L，POD（过氧化物酶）_{················}5KU/L，胆红素氧化酶_{···················}3KU/L，抗坏血酸氧化酶_{·················}3.5KU/L，丙三醇_{·························}4ml/L，聚乙二醇6000_{···················}5g/L，乙二醇_{·························}6ml/L，甘露醇_{·························}25g/L，海藻糖_{·························}15g/L，BSA_{····························}1g/L，烷基糖苷（APG）_{··················}1g/L，防腐剂_{·························}0.5g/L；2）试剂R2的组分为：缓冲液_{·························}100mmol/L，腺苷_{···························}14mmol/L，HTBA（3‑羟基‑2,4,6‑三碘苯甲酸）_··5mmol/L，丙三醇_{·························}4ml/L，聚乙二醇6000_{···················}5g/L，乙二醇_{·························}6ml/L，甘露醇_{·························}25g/L，海藻糖_{·························}15g/L，BSA_{····························}1g/L，烷基糖苷（APG）_{··················}1g/L，防腐剂_{·························}0.5g/L。