Hydrodynamic Methods for Delivering Fluids to Kidney Tissues and Related Materials and Methods

摘要

The present invention provides methods and materials useful delivering liquids, including liquids comprising nucleic acid molecules into cells. In particular, the present invention provides methods for delivering saline solution, exogenous compositions, and isolated vectors to kidney cells, using the renal vein as a guide and under hydrodynamic pressure. The delivery methods and materials herein are useful to research, prognose, ameliorate symptoms of kidney injury, and treat kidney pathologies.

主权项

1. An augmented hydrodynamic method for delivering fluid into at least one kidney cell of a mammalian subject, comprising: administering fluid into at least one kidney of the mammalian subject using the subject's renal vein as a guide for administering the fluid to the at least one kidney, wherein the fluid is administered to the at least one kidney via the renal vein, under retrograde hydrodynamic pressure, and wherein at least one renal blood vessel is temporarily occluded, is partially occluded, or is temporarily partially occluded.