| 摘要 |
An in-vitro process for the quick determination of the infection status of an infection with an influenza virus type from whole blood in terms of an acute influenza infection or a pre-immunized latent influenza infection, comprising the steps of: . stimulating an antigen-specific T cell that is present in a first sample of whole blood with antigens of the influenza virus type, optionally in the presence of antibodies against CD28, or CD28 and CD49d; . processing of the influenza antigens by antigen-presenting cells (APC) by incubation for a sufficient time, in particular by incubation of at least 1 h, particularly of 1.5 h to 2.5 h, especially for 2 h, at 35 °C to 39 °C, especially at 37 °C, optionally with adding CO2; . then adding a secretion inhibitor; . effecting an intensive mixing; . followed by a second incubation step for a period of at least 1 h, in particular 2.5 h, at a temperature of from 35 °C to 39 °C, and . determining a profile from both the intracellular INF-gamma production and the intracellular IL-2 production of the antigen-specific T cell; wherein . the presence of an acute influenza infection is indicated by a shift of the profile towards IFN-gamma single positive cells accompanied by a larger ratio of IFN-gamma/IL-2 single positive T cells compared to the ratio of IFN-gamma/IL-2 single positive T cells of patients successfully vaccinated against, pre-infected or non-infected by the influenza virus. |
