COMPOSITIONS AND METHODS FOR OBTAINING STEM CELL DERIVED LUNG TISSUE, AND RELATED USES THEROF

摘要

The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting definitive endoderm formation from pluripotent stem cells. The invention disclosed herein further relates to methods and systems for promoting ventral-anterior foregut spheroid tissue formation, 3-dimensional lung tissue formation, and lung organoid tissue formation produced in vitro from the described methods

主权项

1. A method, comprising:
culturing definitive endoderm cells in vitro, wherein the culturing results in differentiation of the definitive endoderm cells into tissue comprising ventral-anterior foregut spheroid tissue, wherein the culturing comprises activating and/or inhibiting one or more signaling pathways within the definitive endoderm cells, wherein the one or more signaling pathways are selected from the group consisting of the Wnt signaling pathway, the FGF signaling pathway, the BMP signaling pathway, and the TGFβ signaling pathway; and obtaining ventral-anterior foregut spheroid tissue from the cultured definitive endoderm cells.