| 主权项 |
1. A preparation method of a novel recombinant antibacterial polypeptide medicine comprises the steps as follows:
(1) Preparing Escherichia coli strain comprising recombinant plasmid and freezing for conserving, (2) Enlarging cultivation of the strain in liquid production medium, (3) Inducing the strain to express the recombinant antibacterial polypeptide and getting purified polypeptide, characterized in that, said liquid production medium comprises, in w/v %, disodium hydrogen phosphate 0.4%-0.7%, potassium dihydrogen phosphate 0.1%-0.6%, ammonium chloride 0.05%-0.2%, calcium chloride 0.0005%-0.001%, magnesium sulfate 0.5%-2.5%, peptone 1%-3%, yeast extract powder 0.5%-1%, glucose 0.1%-0.5%, sodium chloride 0.2%-0.8% and water of the rest. |
