| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to clinical laboratory diagnostics and concerns a method of determining cerebral isoform of creatine phosphokinase in human blood. Method comprises determination of activity of general creatine phosphokinase and cardiac isoform of creatine phosphokinase, as well as content of creatine phosphokinase cardiac isoform by weight. Cerebral isoform of creatine phosphokinase is determined by following formula: CPK-VV akt=K×(CPK-MV akt/CPK-MV weight)+K×(CPK-MV akt/CPK COMMON akt)+K×(CPK-MV weight), where CPK-VV akt is activity of cerebral isoform of creatine phosphokinase, units/l; CPK-MV akt is activity of creatine phosphokinase cardiac isoform defined by kinetic method, units/l; CPK-MV mass is amount of creatine phosphokinase cardiac isoform defined immunochemical method, mg/l; CPK COMMON akt is activity of all forms of creatine phosphokinase, including cerebral, cardiac and muscular phosphokinase activity, defined by kinetic method, units/l; K=0.64; K=-32.30; K=2.62 are numerical coefficients obtained using umbilical blood of newborns in sample for analysis, kinetic method for determining CPK COMMON akt, CPK-MV and immunochemical method for determining CPK-MV weight.EFFECT: use of method makes it possible to quickly identify cerebral isoform of creatine phosphokinase in human blood.1 cl, 3 dwg |
