ANTIBODIES FOR DIAGNOSIS OF ACUTE MYELOID LEUKEMIA

摘要

A polypeptide in particular an antibody or antibody fragment is disclosed wherein the polypeptide is corresponding to certain complementarity determining regions CDR1, CDR2 and CDR3 of a heavy chain VH and a light chain VL of an antibody as well as a compound comprising the polypeptide, its use as diagnostic agent for acute myeloid leukemia subtype M2 and a kit comprising the compound.

主权项

1. A polypeptide comprising an antibody or antibody fragment for AML subtype M2 specific diagnosis wherein the polypeptide corresponds to complementarity determining regions CDR1, CDR2 and CDR3 of a heavy chain VH and a light chain VL of an antibody, the complementarity determining regions comprising:
the CDR 1 region of the heavy chain VH defined by a sequence of 5 amino acids wherein the amino acids have side chain polarities and charges at a pH of 7.4, and the amino acids of the amino acid sequence are symbolized by a symbol as represented by a formula
PON/PON/NPN/NPN/PON and the amino acids are linked via peptide bonds;
the CDR 2 region of the heavy chain VH defined by a sequence of 17 amino acids wherein the amino acids have side chain polarities and charges at a pH of 7.4, and the amino acids of the amino acid sequence are symbolized by a symbol as represented by a formula
PON/NPN/PON/PON/BP+ or NPN/PON/BP+ or PON/BP+/PON/NPN/PON/NPN/AP−/PON/NPN/BP+/PON and the amino acids are linked via peptide bonds;
the CDR 3 region of the heavy chain VH defined by a sequence of 7 amino acids wherein the amino acids have side chain polarities and charges at a pH of 7.4, and the amino acids of the amino acid sequence are symbolized by a symbol as represented by a formula
NPN/NPN or BP+/BP+ or NPN/BP+ or PON/NPN/AP−/PON and the amino acids are linked via peptide bonds;
the CDR 1 region of the light chain VL defined by a sequence of 11 amino acids wherein the amino acids have side chain polarities and charges at a pH of 7.4, and the amino acids of the amino acid sequence are symbolized by a symbol as represented by a formula
BP+/NPN/PON/PON/PON/NPN/PON/PON/PON/NPN/PON and the amino acids are linked via peptide bonds;
the CDR 2 region of the light chain VL defined by a sequence of 7 amino acids wherein the amino acids have side chain polarities and charges at a pH of 7.4, and the amino acids of the amino acid sequence are symbolized by a symbol as represented by a formula
NPN or BP+/NPN/PON/BP0 or NPN/NPN/PON/PON and the amino acids are linked via peptide bonds; and
the CDR 3 region of the light chain VL defined by a sequence of 9 amino acids wherein the amino acids have side chain polarities and charges at a pH of 7.4, and the amino acids of the amino acid sequence are symbolized by a symbol as represented by a formula
PON/PON/NPN or BP+/BP+ or NPN/PON or BP+/PON/NPN/NPN/PON and the amino acids are linked via peptide bonds;
wherein the amino acids of the formulas are proteinogenic amino acids and the symbols have the meaning: PON represents an amino acid having a polar side chain polarity and a neutral side chain charge at pH 7.4, NPN represents an amino acid having a non-polar side chain polarity and a neutral side chain charge at pH 7.4, BP+ represents an amino acid having a basic polar side chain polarity and a positive side chain charge at pH 7.4, BP0 represents an amino acid having a basic polar side chain polarity and a predominantly neutral side chain charge at pH 7.4, and AP− represents an amino acid having an acidic polar side chain polarity and a negative side chain charge at pH 7.4.