发明名称 |
RIBOSWITCH INDUCIBLE GENE EXPRESSION |
摘要 |
An intronic, self-splicing riboswitch is configured for enzyme-product specificity by introducing an appropriate aptamer. This then provides a sensing-expression construct, whereby the presence of an enzyme product in the cell triggers self-splicing of the intron sequence to restore the reading frame of the reporter gene and as such to drive expression of the gene product. The sensing construct expresses a protein which marks the cell or permits its growth or survival in or on an otherwise selective media. In this way, introduction or the presence of such product sensing-reporter constructs in cells can be harnessed to provide a multi-parallel rapid screening of cells or libraries for desirable enzyme variants. |
申请公布号 |
WO2016166310(A1) |
申请公布日期 |
2016.10.20 |
申请号 |
WO2016EP58383 |
申请日期 |
2016.04.15 |
申请人 |
WAGENINGEN UNIVERSITEIT |
发明人 |
CREUTZBURG, Sjoerd Constantijn Arnoud;VAN DER OOST, John |
分类号 |
C12N15/10;C12N15/63;C12N15/67;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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