| 摘要 |
To achieve multidimensional high-resolution imaging of a structure of a sample, marked with fluorescent markers, the following steps are repeated for a plurality of measurement regions (18) in said sample: fluorescence-enabling light (3) is focussed into the sample (4), wherein the region illuminated in the sample (4) by the fluorescence-enabling light (3) constitutes the measurement region (18) in question; fluorescence-hindering light (10) is applied to a subsection (20) of the measurement region (18) wherein, since an intensity distribution of the fluorescence-hindering light (10) has a linear or planar zero position (21) that extends across a centre (22) of the measurement region (18), this subsection (20) does not include said centre (22); a minimum extension (23) of the zero position (21) through the centre (22) of the measurement region (18) is smaller, by a factor of k ≥ 2, than a diameter (19) of the measurement region (18) in the same direction; and fluorescent light emitted from the measurement region (18) of the sample is measured without spatial resolution within the measurement region (18) for a plurality of successive angular positions of the zero position (21) around the centre (22), the fluorescence-enabling light (3) being applied to said measurement region (18) for each of the angular positions of the zero position (21). |
