发明名称 |
METHODS FOR INCREASING N-GLYCAN OCCUPANCY AND REDUCING PRODUCTION OF HYBRID N-GLYCANS IN PICHIA PASTORIS STRAINS LACKING ALG3 EXPRESSION |
摘要 |
Methods are disclosed for increasing the yield and N-glycosylation site occupancy of paucimannose or complex N-glycans of recombinant glycoproteins produced in a recombinant host cell lacking dolichyl-P-Man:Man5GlcNAc2-PP-dolichyl alpha-1,3 mannosyltransferase (Alg3p) activity. In particular, the present invention provides recombinant host cells for producing recombinant glycoproteins that comprise a disruption of the expression of an OS-9 family gene in the host cell and which overexpress one or more Trypanosoma brucei STT3 proteins. |
申请公布号 |
EP2956158(A4) |
申请公布日期 |
2016.10.05 |
申请号 |
EP20140752178 |
申请日期 |
2014.02.07 |
申请人 |
MERCK SHARP & DOHME CORP. |
发明人 |
CHOI, BYUNG-KWON |
分类号 |
A61K38/16;A61K38/00;C07K9/00;C07K14/39;C07K14/44;C07K14/62;C07K16/28;C12N9/10;C12P21/00 |
主分类号 |
A61K38/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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