METHODS FOR ASSESSING CANCER

摘要

Provided herein are methods for assessing cancer, comprising analysis of sequence data from a set of cancer-related genes in a tumor sample from a subject, followed by monitoring of a subset of the set in circulating tumor-associated DNA in a fluid sample from the subject. Also provided are kits and systems for practicing any of them methods of the invention.

主权项

1. A method comprising:
a. ligating a single-stranded DNA (ssDNA) fragment to a first single-stranded adaptor by single-stranded ligation to generate a ssDNA fragment attached to said first single-stranded adaptor, wherein said ssDNA fragment attached to said first single-stranded adaptor is a member of a ssDNA library; b. hybridizing a target-selective oligonucleotide (TSO) to said ssDNA fragment attached to said first single-stranded adaptor to create a hybridization product, wherein said TSO comprises
i. a sequence that is selectively complementary to a single target region of said ssDNA fragment, andii. a second single-stranded adaptor sequence located at a first end of said TSO, wherein said second single-stranded adaptor sequence is different than said first single-stranded adaptor; and c. extending said TSO to generate an extended strand comprising said second adaptor sequence, said TSO, a reverse complement of said target sequence, and a reverse complement of said first adaptor sequence.