RNA PREPARATIONS FOR CELL REPROGRAMMING COMPRISING PURIFIED MODIFIED RNA

摘要

PROBLEM TO BE SOLVED: To provide compositions and methods for reprogramming somatic cells using purified RNA preparations comprising a single-stranded mRNA encoding an Induced Pluripotent Stem Cell (iPSC) induction factor.SOLUTION: Preferably the purified RNA preparation is substantially free of contaminant RNA molecules that may: i) activate immune response in somatic cells; ii) decrease expression of single-stranded mRNA in somatic cells; and/or iii) activate RNA sensors in somatic cells. In certain embodiments, the purified RNA preparation is substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules and/or single-stranded run-on mRNAs.SELECTED DRAWING: Figure 1