A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.
申请公布号
EP3072968(A1)
申请公布日期
2016.09.28
申请号
EP20160156020
申请日期
2011.02.22
申请人
ADVANCED LIQUID LOGIC, INC.
发明人
POLLACK, MICHAEL G.;ECKHARDT, ALLEN E.;SUDARSAN, ARJUN;SRINIVASAN, VIJAY;ROUSE, JEREMY;YI, UICHONG;TROTTA, NICHOLAS;DHOPESHWARKAR, RAHUL;THWAR, PRASANNA;SMITH, GREGORY F.;NORTON, SCOTT;LINNARTZ, PETER