| 摘要 |
Object of the present invention is to provide a method for finding a target-molecule-binding polypeptide, from a library constructed by a display method capable of associating a polypeptide with a nucleic acid encoding it, wherein the polypeptide is one that cannot be found by a method of infecting a phage obtained by repeating conventional panning with Escherichia coli and cloning it; and for designing, based on the amino acid sequence or nucleic acid sequence information of the polypeptide, a polypeptide that binds to a target molecule. The present invention provides a method of determining a sequence of a polypeptide that binds to a target molecule, including a step of panning using a target molecule and a library constructed by a display method; a step of analyzing, by a next-generation sequencer, the sequence of all the polypeptides belonging to a polypeptide group of the pre-panning library and all the polypeptides belonging to a polypeptide group of the post-panning library; a step of evaluating and scoring an amplification ratio of each of the polypeptides through the panning based on the results of the sequencing step; and a step of selecting a polypeptide with a high score. |
