| 摘要 |
In the scanning molecule counting method using the light measurement with a confocal microscope or a multiphoton microscope, there is provided a technique enabling improvements in its sensitivity and/or S/N ratio. In the optical analysis technique of detecting an existence of a single particle in a sample solution according to the present invention, the position of a light detection region is moved in the sample solution; the light intensity from the light detection region is measured so that light intensity data will be generated; a first occurrence probability in assuming a first condition that no single particles exist in the light detection region and a second occurrence probability in assuming a second condition that a single particle exists in the light detection region for a time variation of light intensity value on the light intensity data are computed; and a signal indicating each single particle is detected by detecting the time in which the single particle existed in the light detection region based on those occurrence probabilities. |
