FLUOROGENIC AND CHROMOGENIC SUBSTRATE

摘要

The present invention provides near-infrared fluorogenic and/or chromogenic formulations, methods, systems and kits. Upon contacting an azine with an oxidant and a peroxidase, the azine is converted to an azine derivative which is both visibly colored and fluorescent. This invention also provides high sensitive methods to detect biological molecules, showing better sensitivity than chemiluminescence methods. Advantageously, the formulation and reaction is free of luminol or luminol derivatives.

主权项

1. A formulation, the formulation comprising a) a first part and b) a second part:
a) the first part comprising one or more azines; and b) the second part comprising an oxidant.